Production of the recombinant fibronectin-binding protein of Staphylococcus aureus

kim, Doo; Cheong, Cha-ryong; Park, Hee-myong; Han, Hong-ryul

Korean Journal of Veterinary Research 1997;37(4):875-882.

Doo kim¹, Cha-ryong Cheong¹, Hee-myong Park², Hong-ryul Han²

¹Department of Veterinary Medicine, Kangwon National University
²College of Veterinary Medicine, Seoul National University

Staphylococcus aureus의 재조합 fibronectin-binding protein의 생산

김두¹, 정자룡¹, 박희명², 한홍율²

¹강원대학교 축산대학 수의학과
²서울대학교 수의과대학

Abstract

To produce the recombinant fibronectin-binding protein(FnBP) for development of subunit vaccine against Staphylococcus aureus. The fnbp gene was amplified from the chromosomal DNA of S aureus KNU 196 strain using the polymerase chain reaction, and cloned into pGEX-4T-2. Then, the recombinant FnBP fused with glutathione-S-transferase was produced in E coli, purified by affinity chromatography, and identified its antigenicity and immunogenicity by Western blot. The recombinant FnBP produced in this study is considered to have the same property of native FnBP purified from S aureus, and is expected to be useful as a candidate for S aureus subunit vaccine.

Key Words: Staphylococcus aureus, fibronectin binding protein, recombinant