Korean Journal of Veterinary Research 1998;38(1):9-15.
Expression of protein kinase C in the testes of horse
Jae-kwang Jin, Tae-kyun Shin
Department of Veterinary Medicine, Cheju National University
말 정소내 protein kinase C의 발현
진재광, 신태균
제주대학교 농과대학 수의학과
To investigate the involvement of protein kinase C(PKC) isoenzyme in the testes which control spermatogenesis and hormone secretion, we examined cellular distribution of four types of PKC $alpha$, ${eta}I$, ${delta}$ and ${ heta}$ in the horse testes using PKC antisera by western blot analysis and immunohistochemistry. By the western blot analysis, PKC $alpha$ and ${eta}I$ were detected at 82KD, while PKC ${delta}$ and ${ heta}$ were detected at 80KD in the testes of both juvenile and adult horses. In juvenile horse, PKC $alpha$, ${delta}$ and ${ heta}$ except ${eta}I$ were not detected in the cells of the testes, whereas PKC ${eta}I$ was immunoreacted with only in spermatocytes. In adult, PKC $alpha$, ${eta}I$, ${delta}$ and ${ heta}$isoenzymes were localized in interstitial cells of the testes. In the seminiferous tubules, PKC ${eta}I$ is localized in spermatocyte, spermatid and spermatozoa, while PKC ${delta}$ is localized only in spermatids. We suggest that this is a first report to localize PKC in the testes of horse and PKC isoenzymes are upregulated in the cells of horse testes depending on ages. These findings also suggest that certain PKC isoenzyme plays an important role in the signal transduction of spermatogenic cells and interstitial cells in horse testes.
Key Words: protein kinase C, horse, testes, western blot, immunohistochemistry

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