Differential diagnosis among Marek's disease, reticuloendotheliosis and avian leukosis by polymeras chain reaction |
Hwan-woo Seong1, Sun-jung Kim2 |
1National Veterinary Research Institute, Ministry of Agriculture & Forestry 2College of Veterinary Medicine, Seoul National University |
중합효소연쇄반응을 이용한 닭 종양성 질병의 감별진단에 관한 연구 |
성환우1, 김선중2 |
1농림부 수의과학연구소 2서울대학교 수의과대학 |
|
Abstract |
The present study attempted to apply polymerase chain reaction (PCR) to develop a rapid differential diagnosis among Marek's disease, reticuloendotheliosis and avian leukosis. The primers chosen to detect Marek's disease virus (MDV) flank the 132bp tandem direct repeat of the MDV genome. The primers selected for reticuloendotheliosis virus (REV) and avian leukosis virus (ALV) are based on proviral long terminal repeats of spleen necrosis virus and Rous-associated virus-2 genomes, respectively. The specific PCR products of MDV, REV and ALV were observed with each primer and the reaction was not cross-reacted among the viruses. MDV-specific DNA was also amplified from the MDV-induced lymphoma (MDCC-MSB1) but not from the REV-induced tumor and ALV-induced lymphoma (LSCC-1104B1). In addition, proviral DNA of REV from REV-induced tumor and proviral DNA of ALV from ALV-induced lymphoma were also amplified by REV-specific and ALV-specific PCRs, respectively. Therefore these three PCR methods may be used to rapidly differentiate among MDV, REV and ALV-associated tumors in diagnosis. |
Key Words:
Marek's disease, reticuloendotheliosis, avian leukosis, polymerase chain reaction, differential diagnosis |
|