In vitro isolation of a bovine Neospora in Korea

Kim, Jae-hoon; Sohn, Hyun-joo; Hwang, Eui-kyung; Hwang, Woo-suk; Hur, Kwon; Jean, Young-hwa; Lee, Byung-chun; Rhee, Jae-chin; Kang, Yung-bai; Yamane, Itsuro; Kim, Dae-yong

Korean Journal of Veterinary Research 1998;38(1):139-145.

Jae-hoon Kim¹, Hyun-joo Sohn¹, Eui-kyung Hwang¹, Woo-suk Hwang², Kwon Hur², Young-hwa Jean¹, Byung-chun Lee², Jae-chin Rhee¹, Yung-bai Kang¹, Itsuro Yamane³, Dae-yong Kim²

¹National Veterinary Research Institute
²College of Veterinary Medicine, Seoul National University
³Laboratory of Epidemiology, National Institute of Health

국내 소에서 Neospora caninum의 분리

김재훈¹, 손현주¹, 황의경¹, 황우석², 허권², 진영화¹, 이병천², 이재진¹, 강영배¹, 야마네이츠로³, 김대용²

¹수의과학연구소
²서울대학교 수의과대학
³일본 가축위생시험장

Abstract

The Neospora sp. was isolated from the brain of 1 calf via continuous in vitro cultivation in Vero cell. Neospora tachyzoites were observed 45 days after inoculation of the homogenized brain suspension into the Vero cell. The isolated parasite (named tentatively as NCKB-1) was morphologically and ultrastructurally similar to the previously reported Neospora sp isolated in cattle (BPA-1, JPA-1). A comparison of the antigenic reactivity of cultivated tachyzoites with polyclonal antisera to Neospora caninum and Toxoplasma gondii confirmed that this protozoal isolate was similar to N caninum. This is the first report of successful isolation of Neospora sp from cattle in Korea.

Key Words: calf, Vero cell, tachyzoite, Neospora caninum, isolation