Korean Journal of Veterinary Research 1998;38(1):139-145.
In vitro isolation of a bovine Neospora in Korea
Jae-hoon Kim1, Hyun-joo Sohn1, Eui-kyung Hwang1, Woo-suk Hwang2, Kwon Hur2, Young-hwa Jean1, Byung-chun Lee2, Jae-chin Rhee1, Yung-bai Kang1, Itsuro Yamane3, Dae-yong Kim2
1National Veterinary Research Institute
2College of Veterinary Medicine, Seoul National University
3Laboratory of Epidemiology, National Institute of Health
국내 소에서 Neospora caninum의 분리
김재훈1, 손현주1, 황의경1, 황우석2, 허권2, 진영화1, 이병천2, 이재진1, 강영배1, 야마네이츠로3, 김대용2
1수의과학연구소
2서울대학교 수의과대학
3일본 가축위생시험장
Abstract
The Neospora sp. was isolated from the brain of 1 calf via continuous in vitro cultivation in Vero cell. Neospora tachyzoites were observed 45 days after inoculation of the homogenized brain suspension into the Vero cell. The isolated parasite (named tentatively as NCKB-1) was morphologically and ultrastructurally similar to the previously reported Neospora sp isolated in cattle (BPA-1, JPA-1). A comparison of the antigenic reactivity of cultivated tachyzoites with polyclonal antisera to Neospora caninum and Toxoplasma gondii confirmed that this protozoal isolate was similar to N caninum. This is the first report of successful isolation of Neospora sp from cattle in Korea.
Key Words: calf, Vero cell, tachyzoite, Neospora caninum, isolation


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