Studies on practical application of zearalenone ELISA kits

Yoon, Hwa-joong; Kim, Tae-Jong; Lee, Sung-Yun; JeGal, Jun; Yoon, Ji-Byung

Korean Journal of Veterinary Research 1998;38(2):297-303.

Hwa-joong Yoon¹, Tae-Jong Kim¹, Sung-Yun Lee¹, Jun JeGal¹, Ji-Byung Yoon²

¹Department of Veterinary Medicine, College of Animal Husbandry Kon-kuk University
²Choong Ang Animal Disease Laboratory

Zearalenone ELISA kits의 응용에 관한 연구

윤화중¹, 김태종¹, 이승윤¹, 제갈준¹, 윤지병²

¹건국대학교 축산대학 수의학과
²중앙가축전염병연구소

Abstract

For the extraction and measurement of zearalenone in the corn, bean, wheat and barley contaminated with Fusarium graminearum, the zearalenone-oxime, zearalenone-oxime BSA and zearalenone monoclonal antibodies were studied to develop and apply the direct competitive enzyme linked immunosorbent assay (ELISA). The extraction range of zearalenone with the monoclonal antibodies produced in this experiment was 10ng to 500ng/g feed and the 50% inhibition value was 50ng/ml. The mean recoveries of zearalenone artificially spiked in the ground corn were 89%. The specificity of F-2 monoclonal antibody for the analogues was favorable for the direct competitive ELISA. The result of the experiment showed the zearalenone in the corn, bean, wheat and barely naturally contaminated with the mold would be suitable for extraction and measurement with the monoclonal antibodies.

Key Words: Enzyme linked immunosorbent assay (ELISA), zearalenone, zearalanone, cross reaction, monoclonal antibody