Sequence of the spike gene containing antigenic sites A and D of transmissible gastroenteritis virus isolated in Korea |
Hyuk-moo Kwon1, Jae-ho Pi1, Hwan-woo Seong2 |
1Department of Veterinary Medicine, Kangwon National University 2National Veterinary Research Institute |
국내분리 돼지 전염성 위장염 바이러스의 antigenic site A와 D를 포함하는 spike gene의 염기서열 분석 |
권혁무1, 피재호1, 성환우2 |
1강원대학교 수의학과 2수의과학연구소 |
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Abstract |
The nucleotide sequences of spike (S) glycoprotein containing antigenic sites A and D of TGEV isolated in Korea were determined and compared with published sequences for TGEVs. The TGEV 133 and DAE5 strains had 97.40% nucleotide sequence similarity. The overall nucleotide sequence similarity of the 133 and DAE5 strains compared with other TGEV strains was between 96.86% and 99.15%. The similarity of the predicted amino acid sequence of the 133 and DAE5 strains was 94.93%. The TGEV 133 and DAE5 strains had 94.93-98.61% amino acid similarity with published sequences of other TGEV strains. The sequences of amino acid codons in the antigenic sites A and D were identical among all the viruses although there were several nucleotide changes in region containing antigenic sites A and D of Korean TGEV isolates. By phylogenetic analysis of the sequences, two Korean isolates 133 and DAE5 seemed to be derived from different lineages. These studies showed that a distinct difference in genome exists among TGEV field strains isolated in Korea. |
Key Words:
TGEV, coronavirus, spike glycoprotein gene, sequencing |
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