Production and characterization of monoclonal antibodies against porcine transmissible gastroenteritis virus |
Young-en Jang1, Sun-hee Cho1, Byung-han Kim2, Jae-moon Ahn3, Shien-young Kang1 |
1College of Veterinary Medicine, Chungbuk National University 2National Veterinary Research Institute 3Northern Branch of Chungbuk Office of Agriculture and Livestock |
돼지 전염성 위장염 바이러스에 대한 단크론항체 생산 및 특성 |
장영은1, 조선희1, 김병한2, 안재문3, 강신영1 |
1충북대학교 수의과대학 2수의과학연구소 3충청북도 농축산사업소 북부지소 |
|
Abstract |
Eight monoclonal antibodies(MAbs) against the transmissible gastroenteritis virus (TGEV) were produced and characterized. Four of the MAbs were produced against a reference TGEV, Purdue strain(P115) and the others were produced against the Korean vaccine virus, Pyungtaek strain. Only one MAb(5C8) produced against P115 had neutralizing activity and was found to be E2 protein-specific. The other seven MAbs(4E2, 4G8, 5H6, 1F8, 2C6, 5H5, and 3A6) had specificity of nucleocapsid protein and no neutralizing activity. All MAbs reacted with different strains of TGEV, but none of the MAbs was reactive with porcine enteropathogenic viruses such as rotavirus, epidemic diarrhea virus and enterovirus by fluorescence antibody(FA) test. |
Key Words:
transmissible gastroenteritis virus, monoclonal antibody |
|