| Distribution of positive cells by two monoclonal antibodies (MT1, MB2) in rat spleens |
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Soo-dong Kwak, Phil-ok Koh, Chong-sup Kim |
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Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University |
| Rat 비장에서 MT1 과 MB2 항체의 양성반응세포 분포 |
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곽수동, 고필옥, 김종섭 |
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경상대학교 수의과대학, 동물의학연구소 |
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| Abstract |
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This study was designed to investigate the distributions of the positive cells in rat spleens by two monoclonal antibodies of MT1 and MB2. The spleens of immature 10 rats (Sprague Duwely, approximately 200gm) were collected and paraffin-embedded sections of spleens were stained with immunohistochemical methods. Higher proportions of MT1-positive cell number in spleens were ordered as marginal zone(8.5~18.1%), red pulp(2.1~8.8%) and periarterial lymphoid sheath(0~1.6%) in white pulp, and those of MB2-positive cell number are ordered as the central area of the periarterial lymphoid sheath(100%), red pulp(29.1~45.0%), marginal zone(15.2~30.4%), and peripheral area of periarterial lymphoid sheath(2.3~3.5%). The positive cells by MB2 are more numerous in number than by MT1. The above results were concluded that the positive cells by above two monoclonal antibodies were scattered throughout the red pulp and marginal zone, but in the central area of the periarterial lymphoid sheath, the MB2-positive cells only were present. |
| Key Words:
MT1, MB2, Immuno stain, spleen, rat |
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