| Gene typing of staphylococcal superantigens produced by Staphylococcus aureus isolates from meat |
|
Jang-won Yoon1, Suk-chan Jung2, Soo-jin Yang1, Byong-youel Jung2, Keon-suk Seo1, So-hyun Kim1, Yong-ho Park1 |
1Department of Microbiology, College of Veterinary Medicine, Seoul National University
2National Veterinary Research and Quarantine Services |
| 식육유래의 Staphylococcus aureus으로부터 산생되는 staphylococcal superantigens의 유전자형 분석 |
|
윤장원1, 정석찬2, 양수진1, 정병열2, 서근석1, 김소현1, 박용호1 |
1서울대학교 수의과대학 미생물학교실
2농림부 국립수의과학검역원 |
|
|
| Abstract |
|
Using the previous established multiplex PCR (mPCR), the presence of six kinds of staphylococcal superantigen (SAg) genes was investigated for nineteen Staphylococcus aureus isolates from the commercialized meat sources. As a result, only one isolate from pork among 19 S aureus isolates (5.3%) was confirmed as a potential SAg producer and harbored sec gene. The results in this study suggest that meat may not be major contagion of staphylococcal food poisoning (SFP) in Korea and that staphylococcal enterotoxin type C may be associated with the disease. Also, the mPCR method in this study can be a useful genotypic method which can overcome the typical disadvantages of conventional antibody-based methods due to antigenic homology, and furthur survey on food-borne S aureus isolates can provide the important epidemiological data for SFP in Korea. |
| Key Words:
staphylococcal enterotoxins, Toxic shock syndrome toxin-1, Multiplex PCR, meat |
|
PDF Links
Full text via DOI
Download Citation
