| Rapid diagnosis of experimental listeriosis in mice by polymerase chain reaction |
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Ho-jo Kang1, Seong-mi Lee1, Ju-myoung Suk2, Deog-kyu Lee3, Won-geun Son4 |
1College of Veterinary Medicine Gyeonsang National University
2Institute of Animal Medicine, Gyeongsang Natinal University
3Masan Junior College
4Health Canada, Animal Disease Research Institute, Canadian Food Inspection Agency |
| 중합효소연쇄반응을 이용한 실험적 리스테리아 감염증의 신속진단 |
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강호조1, 이성미1, 석주명2, 이덕규3, 손원근4 |
1경상대학교 수의과대학
2경상대학교 동물의학연구소
3마산전문대학 방사선과
4캐나다 식품검사국 |
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| Abstract |
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The polymerase chain reaction(PCR) assay was used for rapid diagnosis from blood and organ samples experimentally infected with Listeria monocytogenes. This method used a pair of primers based on a unique region in the 16S rRNA sequence of L monocytogenes. Procedure A was based on dilution of the blood sample followed by lysis of bacterial cell and direct analysis of the lysate with PCR. In artificially infected blood samples with L monocytogenes, it was possible to detect fewer than 40 cells per ml of blood. However, L monocytogenes was detected low rates on infected organs by the direct PCR. In procedure B, enrichment cultivation was used to increase numbers of bacteria before lysis and PCR. L monocytogenes was detected from 23 samples of 24 liver and spleen, respectively, and 18 samples of 24 blood were found to be positive by PCR on a subset of 72 organ samples, whereas L monocytogenes were detected on 63 organ samples in classical culture technique. It was required to analyze including enrichment steps were 6h and 18h on the procedure A and B, respectively. |
| Key Words:
Listeria monocytogenes, experimental listeriosis, polymerase chain reaction, rapid diagnosis |
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