| Establishment of PCR to detect Bacillus anthracis in the experimentally infected soil and mice |
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Ji-youn Lee1, Han-sang Yoo2, Jong-yeom Kim1 |
1Department of Bacteriology and Immunology, National Veterinary Research and Quarantine Services, MAF
2Department of Infectious Disease, College of Veterinary Medicine, Seoul National University |
| PCR 기법을 이용한 인공감염토양 및 감염동물 장기로 부터 Bacillus anthracis의 검출 |
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이지연1, 유한상2, 김종염1 |
1국립수의과학검역원
2서울대학교 수의과대학 |
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| Abstract |
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Anthrax caused by Bacillus anthracis is one of the most important zoonotic diseases in the worldwide. To control and prevent the disease effectively, several methods such as development of a fast and specific diagnostic method and vaccine, education etc, have been carried out. However, it still has a problem in the control and prevention. To control, the most important method is the prevention of direct or indirect contact of the causative agent with susceptible host. Therefore, we developed a fast and specific detection method, polymerase chain reaction, of B anthracis from soil and infected animals because the organism could survive long time in the environment including soil due to formation of spore. With the method, virulence genes of B anthracis were successfully amplified from experimentally infected soil and mice. Up to $4.2{ imes}10$ of the organisms per gram could be detected with the PCR method from experimentally infected soil. These results suggested that this PCR method could be effectively used not only to detect B anthracis in soil and infected animal but also to provide the information to prevent the disease. |
| Key Words:
PCR, B anthracis, soil, mice |
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