Production and characterization of monoclonal antibody against bovine coronavirus

Ahn, Jae-moon; Kang, Shien-young

Korean Journal of Veterinary Research 1998;38(3):581-588.

¹Northern Branch of Chungbuk Office of Agriculture and Livestock
²College of Veterinary Medicine, Chungbuk National University

소 코로나바이러스에 대한 단크론항체 생산과 특성

안재문¹, 강신영²

¹충청북도 농축산사업소 북부지소
²충북대학교 수의과대학

Abstract

Eight monoclonal antibodies(MAbs) against bovine coronavirus(BCV) were produced and characterized. Three MAbs(1G9, 4H12, 5C1) specific to the S glycoprotein and two HE glycoprotein-specific MAbs(2A5, 5G4) were found to neutralize the BCV in fluorescence focus neutralization(FFN) test. Two HE-specific MAbs from the neutralizing MAbs inhibited the hemagglutinating activity of the BCV. None of the N protein-specific MAbs(1C1, 5A12, 6H1) neutralized the virus infectivity. Bovine coronavirus and mouse hepatitis virus, which belong to group II coronaviruses, were differentiated from other groups of coronaviruses(porcine transmissible gastroenteritis virus, porcine epidemic diarrhea virus, canine coronavirus) by all MAbs in fluorescence antibody test(FA), but not in FFN test.

Key Words: bovine coronavirus, monoclonal antibody