| Effect of purinoceptor to perivascular nerve stimulation on isolated coronary artery of pig |
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Seok-cheol Jeon1, Cheol-soo Shim2, Joo-heon Kim3 |
1Cardiac Center Masan Samsung General Hospital
2Eastern Branch Gyeongnam Livestock Promotion Institute
3College of Veterinary Medicine and Institute of Animal Medicine Gyeongsang National University |
| 돼지 적출 심관상동맥에 있어서 혈관주위 신경자극에 의한 purinoceptor의 효과 |
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전석철1, 심철수2, 김주헌3 |
1마산삼성병원 심장센타
2경남축산진흥연구소 동부지소
3경상대학교 수의과대학, 동물의학연구소 |
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| Abstract |
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To elucidate whether or not innervation of purinergic nerve and functional action of $P_{2X}$-, $P_{2Y}$-purinoceptor, the neurogenic effects of perivascular nerve stimulation were investigated using by physiograph recording system in isolated coronary artery of pig. 1. The contractile responses induced by perivascular nerve stimulation (20V, 0.5msec, 20sec) were the frequency (2~64Hz) dependent manner. 2. The neurogenic contractile responses induced by perivascular nerve stimulation were inhibited by the pretreatment with either ATP or adenosine ($10^{-7}{sim}10^{-4}M$). 3. The neurogenic contractile responses induced by perivascular nerve stimulation (20V, 16Hz, 0.5msec, 20sec) were increased by the pretreatment with reactive blue 2, but were not affected by the pretreatment with 8-phenyltheopylline ($10^{-5}M$). 4. The neurogenic contractile responses induced by perivascular nerve stimulation (20V, 16Hz, 0.5msec, 20sec) were inhibited by the desensitization of the P2X-purinoceptor using by treatment of $10^{-5}M$, -methylene ATP as 3 times over again. The accomplished present study on isolated coronary artery of pig suggest that purinergic nerve is innervated and that the neurogenic contractile response was mediated by activation of $P_{2X}$-purinoceptor and the neurogenic relaxative response was mediated by activation of both $P_1$ and $P_{2Y}$-purinoceptor. |
| Key Words:
purinergic, $P_{2X}$-purinoceptor, $P_{2Y}$-purinoceptor, perivascular nerve stimulation, coronary artery, pig |
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