Polymerase chain reaction for a rapid and specific identification of Streptococcus suis

Jung, Byeong-yeal; Jung, Suk-chan; Kim, Jong-yeom; Park, Yong-ho; Kim, Bong-hwan

Korean Journal of Veterinary Research 1998;38(4):771-776.

Byeong-yeal Jung¹, Suk-chan Jung¹, Jong-yeom Kim¹, Yong-ho Park², Bong-hwan Kim³

¹Department of Bacteriology, National Veterinary Research and Quarantine Service, MAF
²College of Veterinary Medicine, Seoul National University
³College of Veterinary Medicine, Kyungpook National University

Streptococcus suis 신속동정을 위한 PCR 기법

정병열¹, 정석찬¹, 김종염¹, 박용호², 김봉환³

¹농림부 수의과학검역원 세균과
²서울대학교 수의과대학
³경북대학교 수의과대학

Abstract

Synthetic oligonucleotide primers of 20 and 21 bases, respectively, were used in the polymerase chain reaction (PCR) to amplify a sequence of the mrp gene, which encodes the muramidase released protein of Streptococcus suis. Amplification was not recorded when 5 other streptococcal species were tested or when 9 different nonstreptococcal species were tested. A DNA fragment of 517bp was amplified from the genomic DNA of S suis. The lower detection limit was 100pg of the genomic DNA. The primers recognized 34 serotypes of S suis reference strains and 9 isolates from pneumonic lung, brain, nasal discharge, tonsil. This results suggest that the amplification of the mrp gene by PCR method is potential for the identification of S suis isolates.

Key Words: Streptococcus suis, mrp, PCR