Identification and sequence analysis of small subunit ribosomal RNA gene of bovine Theileria isolates from Korea and Japan

Chae, Joon-seok; Park, Jin-ho; Kwon, Oh-deog; Waghela, Suryakant D.; Holman, Patricia J.; Wagner, Gerald G.; Lee, Joo-mook

Korean Journal of Veterinary Research 1998;38(4):909-917.

Joon-seok Chae¹, Jin-ho Park², Oh-deog Kwon², Suryakant D. Waghela¹, Patricia J. Holman¹, Gerald G. Wagner¹, Joo-mook Lee²

¹Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station
²College of Veterinary Medicine, Chonbuk National University

한국과 일본 소에 감염된 Theileria 분리주의 small subunit ribosomal 유전자의 동정 및 분석

채준석¹, 박진호², 권오덕², ¹, ¹, ¹, 이주묵²

¹미국 텍사스 A&M 대학교 수의과대학
²전북대학교 수의과대학

Abstract

Six different sequences types(A through E and H) and a subtype(Bl) of the small subunit ribosomal RNA(SSUrRNA) gene were found in bovine Theileria isolates from different areas of Korea and Japan. The sequences were aligned and three hypervariable regions were observed in the nucleotide position ranges 212~231, 261~270 and 632~690. Five of the Theileria isolates yielded sequence type A; these were the field isolates KCB, KCN, and KCJ, and the laboratory stock KLS, all from Korea, and a single isolate from Japan (JHS). This sequence type is identical to the SSUrRNA gene sequence listed for Theileria buffeli (GenBank Accession No. Z15106) from Marula, Kenya. The Korean field isolate KKB yielded only a single sequence type (B), but multiple sequence types were found in some isolates. For example, KCB and JHS isolates yielded both types A and B ; isolate KKW showed types B and H; isolate KCN showed types A, C, and D ; and isolate KCJ showed types A, B, E, and a subtype B1. Finding of the multiple sequences SSUrRNA gene sequences suggests that bovine Theileria isolates from both Korea and Japan may consist of mixed populations.

Key Words: Theileria spp., bovine, small subunit ribosomal RNA, PCR, sequencing