| Immunohistochemical study of neurotensin-, pancreatic polypeptide- and gastrin/cholecystokinin-immunoreactive cells in the gastrointestinal tract of the bullfrog, Rana catesbeiana during developmental stages |
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Hyeung-sik Lee1, Sae-kwang Ku2, Jae-hyun Lee2 |
1Faculty of Basic Science, College of Natural Science, Kyungsan University
2Laboratory of Histology, College of Veterinary Medicine, Kyungpook National University |
| 개체발생에 따른 황소개구리(Rana catesbeiana) 위장관에서 neurotensin, pancreatic polypeptide 및 gastrin/cholecystokinin 면역반응세포에 대한 면역조직화학적 연구 |
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이형식1, 구세광2, 이재현2 |
1경산대학교 자연과대학 기초과학부
2경북대학교 수의과대학 조직학교실 |
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| Abstract |
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To investigate the regional distribution and relative frequency of the neurotensin-, pancreatic polypeptide(PP)- and gastrin/cholecystokinin(Gas/CCK)-immunoreactive cells in the gastrointestinal tract of the bullfrog(Rana catesbeiana) with developmental stages, group of bullfrogs subdivided into the tadpole with hindlegs, metamorphosed bullfrog with tail, 2 weeks after metamorphosed bullfrog and adult bullfrog, were stained by immunohistochemical methods (PAP methods). Neurotensin-immunoreactive cells were observed from the pylorus of the metamorphosed bullfrog with tail, but these cells were not detected after that periods. PP-immunoreactive cells were detected from the adult bullfrog in the pylorus, duodenum and ileum. These cells were most predominant in the pylorus. Gas/CCK-immunoreactive cells were observed from the adult bullfrog in the pylorus. According to these results, most of immunoreactive cells in the gastrointestinal tract of the bullfrog were appeared after the complete metamorphosed periods, in which the complete differentiation of structure of gastrointestinal tract were occurred, and variable changes of the regional distribution and relative frequency with developmental stages were observed. |
| Key Words:
Rana catesbeiana, gastrointestinal tract, endocrine cells, immunohistochemistry |
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