Korean Journal of Veterinary Research 1999;39(1):90-95.
Detection of Mycoplasma gallisepticum using Polymerase Chain Reaction(PCR)
Young-ju Lee1, Ki-seuk Kim1, Jong-wan Kim1, Ryun-bin Tak2
1National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forest
2College of Veterinary Medicine Kyungpook National University
PCR 기법을 이용한 Mycoplasma gallisepticum의 검출
이영주1, 김기석1, 김종완1, 탁연빈2
1농림부 국립수의과학검역원
2경북대학교 수의과대학
A species-specific 760 base pair(bp) BamHI to EcoRI DNA fragment(fMG-2) of lipoprotein gene was isolated from a Mycoplasma gallisepticum(M gallisepticum) genomic library. Based on the DNA sequence data of fMG-2, a pair of 25bp primers was synthesized. When used in the polymerase chain reaction(PCR), 732bp DNA products were amplified from 6 standard strains and 10 field isolates of M gallisepticum, but not from 2 Mycoplasma synoviae and 7 other Mycoplasma species. The lower detection limit was 100fg of the genomic DNA. Identity of the PCR products was confirmed by comparison of patterns of restriction endonuclease analysis with AseI, DraI, EcoRV and SspI.
Key Words: Mycoplasma gallisepticum, polymerase chain reaction, DNA

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