Establishment of a diagnostic method for porcine proliferative enteropathy using polymerase chain reaction

Lym, Suk-kyung; Lee, Hee-soo; Woo, Sung-ryong; Yoon, Soon-seek; Moon, Oun-kyong; Lee, Yoo-young; Koh, Hong-bum

Korean Journal of Veterinary Research 1999;39(1):118-125.

Suk-kyung Lym¹, Hee-soo Lee¹, Sung-ryong Woo¹, Soon-seek Yoon¹, Oun-kyong Moon¹, Yoo-young Lee¹, Hong-bum Koh²

¹National Veterinary Research & Quarantine Service, Ministry of Agriculture & Forestry
²College of Veterinary Medicine, Chonnam National University

중합효소연쇄반응을 이용한 돼지 증식성 장염 진단기법 확립

임숙경¹, 이희수¹, 우승룡¹, 윤순식¹, 문운경¹, 이유영¹, 고홍범²

¹농림부 국립수의과학검역원
²전남대학교 수의과대학

Abstract

Porcine Proliferative Enteropathy(PPE) is an infectious enteric disease and a major cause of economic loss in swine industry due to weight loss, poor growth and sudden death in growing and finishing pigs at 6 to 20 weeks of age. PPE has been diagnosed by clinical signs, syndrom and lesions in the intestine in Korea. However, the diagnostic method had several problems in the detection of infected or carrier pigs. Therefore, in this study, we established the polymerase chain reaction(PCR) which was a fast, specific and sensitive method for identification of Lawsonia intracellularis (L intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect 1ng of genomic DNA as a template. Identity of the PCR products was confirmed by comparison of pattern of restriction endonuclease analysis with restriction enzyme Hae III and Pst I. Also, the PCR method was applicable to the naturally affected pigs with PPE. Based on the results from this study, the PCR method could be used as a fast and specific diagnostic tool for PPE.

Key Words: porcine proliferative enteropathy(PPE), diagnosis, PCR