Activation of swine plasminogen by staphylokinase of Staphylococcus hyicus subsp. hyicus

Park, Cheong-kyu; Jang, Eun-hee

Korean Journal of Veterinary Research 1999;39(1):126-132.

Cheong-kyu Park, Eun-hee Jang

College of Veterinary Medicine, Kyungpook National University

Staphylococcus hyicus subsp. hyicus의 staphylokinase에 의한 돼지 plasminogen의 활성효과

박청규, 장은희

경북대학교 수의과대학

Abstract

Swine plasminogen is not activated by staphylokinase of Staphylococcus aureus. In this study, the activation of swine plasminogen by staphylokinase of Staph hyicus subsp. hyicus was investigated and the effect of EDTA(disodium) on plasminogen activation was also studied. When the activation of swine plasminogen by staphylokinase of Staph hyicus subsp. hyicus was examined in fresh swine plasma, swine plasminogen could be weakly activated. However, when EDTA was added to the swine plasma, plasminogen activation was markedly enhanced, but this enhancement was not observed on bovine fibrin-dog plasminogen agar plate containing EDTA. Chicken and bovine plasminogens were not activated by staphylokinase of Staph hyicus subsp. hyicus. Using fresh swine plasma agar containing 0.07% EDTA, staphylokinase activity was detected in 96.3% of Staph hyicus subsp. hyicus strains isolated from pigs and in none of the chicken and bovine strains.

Key Words: Staphylococcus hyicus subsp. hyicus, swine plasminogen, staphylokinase, EDTA