| Paternity test in dogs by microsatellite allele analysis |
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Young-jin Chae, Dong-keon Kim, Hana Kim, Moon-han Lee, Woo-suk Hwang, Byoung-chun Lee, Hwa-young Youn, Hang Lee |
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College of Veterinary Medicine, Seoul National University |
| Microsatellite 대립유전자 분석을 통한 개에서의 친자감별 |
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채영진, 김동근, 김하나, 이문한, 황우석, 이병천, 윤화영, 이항 |
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서울대학교 수의과대학 |
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| Abstract |
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Microsatellite allele analysis has been used for individual identification and paternity test. In the present study, the biological father of three puppies was determined by using microsatellite allele amplification analysis. The mother bitch of the litter was a Poongsan dog. The three stud dogs that could have inseminated the bitch, by being in the same residence, were a white Poosan dog, a mixed breed, and a white Jindo dog. DNA was obtained from all the relevant dogs by buccal swabbing. Four loci of tetranucleotide repeat microsatellite were PCR-amplified, and analyzed by polyacrylamide gel electrophoresis and silver staining. The results of genotyping unambigously assigned the Poongsan dog as the biological father. There was no evidence of superfecundation. Therefore, the present study demonstrated the usefulness of microsatellite allele analysis as a simple, efficient method of paternity test in dogs. |
| Key Words:
paternity test, dogs, microsatellite, PCR, silver staining |
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