| Epidemiological analysis of Escherichia coli O157 : H7 by pulsed-field gel electrophoresis and multiplex polymerase chain reaction |
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Byeong-yeal Jung1, Suk-chan Jung1, Dong-hee Cho1, Jong-yeom Kim1, Bong-hwan Kim2 |
1Department of Bacteriology, National Veterinary Research and Quarantine Service, MAF
2College of Veterinary Medicine, Kyungpook National University |
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| Abstract |
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Twenty three strains of Escherichia (E) coli O157 : H7 isolated from Korea, Japan, USA were analyzed by pulsed-field gel electrophoresis (PFGE) of XbaI-digested chromosomal DNA and multiplex polymerase chain reaction. Various PFGE patterns of E. coli O157 : H7 were found on the same farm. Most of the E, coli O157 : H7 strains had shiga-like toxin (slt) II gene only (43.5%) or both slt I and slt II genes(30.4%). eaeA gene was highly conserved in the E. coli O157 : H7. There was no correlation between PFGE and slt gene patterns. The results indicate that various genotypes of E. coli O157 : H7 have spread throughout the country and genomic DNA patterns generated by PFGE are highly specific for different strains and have significant value in epidemiologic investigations of infectious disease outbreaks. |
| Key Words:
E. coli 0157 : H7, pulsed-field gel electrophoresis, multiplex polymerase chain reaction |
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