Korean Journal of Veterinary Research 1999;39(2):338-342.
Epidemiological analysis of Escherichia coli O157 : H7 by pulsed-field gel electrophoresis and multiplex polymerase chain reaction
Byeong-yeal Jung1, Suk-chan Jung1, Dong-hee Cho1, Jong-yeom Kim1, Bong-hwan Kim2
1Department of Bacteriology, National Veterinary Research and Quarantine Service, MAF
2College of Veterinary Medicine, Kyungpook National University
Abstract
Twenty three strains of Escherichia (E) coli O157 : H7 isolated from Korea, Japan, USA were analyzed by pulsed-field gel electrophoresis (PFGE) of XbaI-digested chromosomal DNA and multiplex polymerase chain reaction. Various PFGE patterns of E. coli O157 : H7 were found on the same farm. Most of the E, coli O157 : H7 strains had shiga-like toxin (slt) II gene only (43.5%) or both slt I and slt II genes(30.4%). eaeA gene was highly conserved in the E. coli O157 : H7. There was no correlation between PFGE and slt gene patterns. The results indicate that various genotypes of E. coli O157 : H7 have spread throughout the country and genomic DNA patterns generated by PFGE are highly specific for different strains and have significant value in epidemiologic investigations of infectious disease outbreaks.
Key Words: E. coli 0157 : H7, pulsed-field gel electrophoresis, multiplex polymerase chain reaction


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