| Distribution of the neuropeptide Y immunoreactive neurons in the olfactory bulb of striped field mouse(Apodemus agrarius) |
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Young-gil Jeong1, Kil-soo Kim2, Ju-young Jung3, Nam-seob Lee1, Kyeng-youl Lee3, Moo-kang Kim3 |
1Department of Anatomy, College of Medicine, Konyang University
2Department of Laboratory Animal Research, ASAN Institute for Life Sciences
3College of Vetrinary Medicine, Chungnam National University |
| 야생등줄쥐(Apodemus agrarius) 후각망울의 neuropeptide Y 면역반응세포의 분포 |
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정영길1, 김길수2, 정주영3, 이남섭1, 이경렬3, 김무강3 |
1건양대학교 의과대학 해부학교실
2아산생명과학연구소 실험동물연구실
3충남대학교 수의과대학 |
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| Abstract |
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This study was carried out to investigate the NPY-immunohistochemical characteristics of the olfactory bulb in the striped field mouse(Apodemus agrarius). The animals were anesthesized with thiopental sodium and perfused with 4% paraformaldehyde through left ventricle and aorta. Brains were removed and tranfered 10%, 20% and 30% sucrose. Sections were then cut on a cryostat into $40{mu}m$-thick. The tissue immunostained with avidin-biotinylated complex method. The main olfactory bulb consisted of seven circumferential laminae : an olfactory nerve fiber layer, a glomerular layer with glomeruli surrounding by periglomerular cells, an external plexiform layer having granule and tufted cells, a mitral cell layer, a narrow internal plexiform layer, a granule cell layer forming several cell rows and a layer of white matter. The accessory olfactory bulb had four layers : an olfactory or vomeronasal nerve fiber layer, a glomerular layer consisting of small glomeruli, a mixed layer not distinguishing the external plexiform/mitral cell/granule cell layers and a granule cell layer. Most of NPY-immunoreactive(NPY-IR) neurons in main olfactory bulb were localized in the deeper portion of granule cell layer, white matter and anterior olfactory nucleus. In addition, some NPY-IR neurons were identified in the external plexiform layer. The shape of NPY-IR neurons of all olfactory bulb were predominant round or oval, sometime multipolar in shape. And most NPY-IR processes were parallel to long axis of white matter. In accessory olfactory bulb, NPY-IR neurons were not found in all region. |
| Key Words:
NPY, olfactory bulb, Apodemus agrarius, immunohistochemistry |
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