Korean variant dogs have red blood cells with high amino acid accumulation |
Hiroshi Fujise1, Yoon-kyu Lim2, Kyoung-kap Lee3, Seong-hee Kim2, Woong Hahn2, Gon-hyung Kim3, Ji-hyun Kim3, Young-su Ko3, Sung-sun Kim3, Hwa-young Youn4, Sin-keun Youn5, Yong-soon Lee6, Hideharu Ochiai7, Yasuharu Tanaka1 |
1Laboratory of Pathobiochemistry, School of Veterinary Medicine, Azabu University 2Laboratory of Veterinary Public Health, College of Agriculture, Cheju National University 3Laboratory of Veterinary Internal Medicine, College of Agriculture, Cheju National University 4Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University 5Department of Zoology, Samyook University 6Department of Public Health, College of Veterinary Medicine, Seoul National University 7Laboratory of Pathobiochemistry, Institute of Biosciences, Azabu University |
|
Abstract |
Amino acids, Na, K and glutathione (GSH) in red blood cells (RBCs) and hematological indices were examined in Korean dogs. A total of seven dogs possessing RBCs with high K and high glutathione (GSH)(HK/HG) were found in 42 Korean dogs : three from Cheju dog, two from Jindo dog and two from Korean mongrel. The RBCs in Korean HK/HG dog contained abnormally high aspartate (Asp), Glu and glutamine (Gln) the same as in HK/HG RBCs from Japanese Shiba dog. Two dogs possessing RBCs with HK and low GSH (HK/LG) were found in Cheju dog, and they accumulated Asp and Gln. Thus, not only the existence of HK dog was confirmed in Korean dogs, but HK/LG dog was also found. The Asp concentration in RBCs from seven of 33 LK dogs was more than $1000{mu}mol/lc$, the same as in variant LK RBCs with defective Glu/Asp transport (LK/GAT), while it was less than $800{mu}mol/lc$ in normal LK RBCs. Thus, there were variant dogs having RBCs with abnormally high amino acids accumulation among HK and LK Korean dogs. |
Key Words:
aspartate, dog, high K, glutamate, red blood cells |
|