| Development of needle guidance system and its evaluation for ultrasound-guided follicular aspiration |
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Min-cheol Choi1, Hyo-jong Lee2, Seong-keun Cho3, Tae-yung Kang2, Hyun-hui Won2 |
1College of Veterinary Medicine, Seoul National University
2College of Veterinary Medicine, Gyeongsang National University
3College of Agriculture, Gyeongsang National University |
| 초음파유도 난포채란기의 개발과 이의 검증 |
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최민철1, 이효종2, 조성근3, 강태영2, 원현희2 |
1서울대학교 수의과대학
2경상대학교 수의과대학
3경상대학교 농과대학 |
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| Abstract |
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This study was carried out to develop a newly designed ovum pick-up(OPU) instrument for ultrasound-guided transvaginal follicular aspiration in cows. This new instrument consists of out- & inner-layer stainless pipes and a grip with a trigger(hand) switch. Some gauge types of disposable needles and tubes can be attached to this inner pipe. With this instrument, while grasping an ovary with one hand, the other hand can handle in apiration and vacuum on/off with the least assitant's help. With this instrument the mean recovery rate of bovine follicular oocytes was 45.2%. In recovered oocytes, usable oocytes(Grade I & II) were 30.4% and this rate meant 1.4 oocytes per ovary. For 30 days after initial aspiration with this instrument, some adverse effects such as adhesion, hemorrhage, hematoma and other mass formation in/with ovaries were also examined by rectal examination, ultrasonographic and endoscopic images. Adhesion was found in one ovary 1 week after aspiration, and hemorrhagic lesion was found 1-2 days and petechia were found 3-5 days after aspiration and there was no remarkable adverse effects. It was found that this instrument could be applicable and safe for ovum pick-up in cows. |
| Key Words:
ultrasound-guided follicular aspiration, ovum pick-up(OPU), cows, needle guidance system |
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