| The change of dopaminergic immunoreactive cells in telencephalon and diencephalon of mongolian gerbil by water deprivation |
|
Chi-won Song1, Kyoung-youl Lee1, Il-kwon Park1, Ju-young Jung1, Hyo-jung Kwon1, Chul-ho Lee2, Byung-hwa Hyun2, Geun-jwa Lee3, Woon-jae Song4, Young-gil Jung5, Kang-iee Lee6, Moo-kang Kim1 |
1College of Veterinary Medicine, Chungnam National University
2Korea Research Institute of Bioscience and Biotechnology
3Chungnam Provincial Government Institute of Health and Environment
4Taejon Provincial Government Institute of Health and Environment
5College of Medicine, Konyang University
6College of Oriental Medicine, Taejon University |
| 절수에 의한 mongolian gerbil 종뇌 및 간뇌에서 dopamine성 면역반응세포의 분포변화 |
|
송치원1, 이경열1, 박일권1, 정주영1, 권효정1, 이철호2, 현병화2, 이근좌3, 송운재4, 정영길5, 이강이6, 김무강1 |
1충남대학교 수의과대학 조직학교실
2생명공학연구소
3충남보건환경연구원
4대전보건환경연구원
5건양대학교 의과대학 해부학교실
6대전대학교 한의과대학 |
|
|
| Abstract |
|
Nowadays, mongolian gerbil is notably utilized for the research of brain and water deprivation because of a congenital incomplete willis circle structure in the brain, audiogenic seizure in low noise, and special cholesterol metabolism without water absorption for a long time. In this study, we are intend to identify the morphological changes of the catecholaminergic neuron of brain according to the time lapse in the condition of long term water deprivation. 55 mongolian gerbil were divided 10 groups(control, 1, 2, 3, 4, 5, 10, 15, 20, 42th day water deprivation group), of which each group include 5 mongolian gerbils and 5 normal mongolian gerbils in control group were also used for brain atlas as a control. The brains were observed by the immunohistochemical stain using the TH, DBH and PMNT antibody. The results were as followings; 1. The nerve fibers of the TH-immunoreactive neuron were observed only in the and corpus striatum of the telencephalon. 2. Intensity of the immunostain of the nerve fiber in the cerebral cortex and corpus striatum was decreased gradually day by day after water deprivation. 3. The TH-immunoreactive nerve cells were observed in the paraventricular and periventricular nucleus of the 3rd ventricular in the hypothalamus of mongolian gerbil but the number of nerve cells were decreased from the first day of the water deprivation to the 10th day and increased until the 20th day, after than redecreased from the 20th day by the continuous water deprivation. The number of nerve fibers in this area were increased in the first day, but decreased from the 2nd day of water deprivation. The shape and density of the dopamine secreting cells in the brain of mongolian gerbil by the immunoreactive stain were changed in the continuous water deprivation. In this results, we can conclude that dopamine concerned in the water metabolism of mongolian gerbil, and mongolian gerbil could be used as an animal model for the research of water deprivation. |
| Key Words:
mongolian gerbil, water deprived, dopamine, TH, DBH, PNMT |
|
PDF Links
Full text via DOI
Download Citation
