| Development of competitive enzyme linked immunosorbent assay for detection of Coxiella burnetii antibody in animal |
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Dong-hee Cho1, Yong-ju Kim1, Sung-hwan Wee1, Mi-young Cho1, Chang-hee Kweon1, Yung-bai Kang1, Yong-ho Park2, Sang-nae Cho3 |
1National Veterinary Research and Quarantine Service
2College of Veterinary Medicine, Seoul National University
3College of Medicine, Yonsei National University |
| 동물에서 Coxiella burnetii 항체를 진단하기 위한 경쟁효소면역법 개발 |
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조동희1, 김용주1, 위성환1, 조미영1, 권창희1, 강영배1, 박용호2, 조상래3 |
1국립수의과학검역원
2서울대학교 수의과대학
3연세대학교 의과대학 |
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| Abstract |
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Coxiella burnetii (C burnetii) is the causative agent of Q fever in animal and human. The distribution of the disease has been documented around world. In this study we developed the competitive enzyme linked immunosorbent assay(cELISA) and compared it with indirect immunofluorescent assay(IFA). A monoclonal antibody(Mab) against C burnetii and a peroxidase-conjugated anti-mouse IgM were used as an indicator system competing against antibody in animal serum or as an indicater of the absence of antibody. Sera were considered antibody positive when the percentage inhibition index(PI index) is upper than 30. PI index is calculated as 100-[sample OD/Mab OD)${ imes}100$]. Among 162 bovine serum samples, 23 samples were antibody positive both in cELISA and IFA. And 156 samples showed same results. From goat with experimentally induced infection with C burnetii the antibody was detected 20 days early in cELISA compared to IFA. On the basis of present findings, it was demonstrated that cELISA is a reliable diagnostic method for The detection of specific antibodies against C burnetii infection. |
| Key Words:
Coxiella burnetii, cELISA, monoclonal antibody |
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