| The histological and immunohistochemical effects of acua-acupuncture of Bletillae rhizoma extract on the duodenal ulcer induced by HCl-aspirin |
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Seo-kwang Ku1, Hyeung-sik Lee2, Jong-dae Kim3, Jae-hyun Lee4 |
1Pharmacology & Toxicology Laboratory, Central Research Laboratories, Dong-Wha Pharm Ind. Co.
2Department of Biology, Faculty of Natural Secience, Kyungsan University
3College of Oriental Medicine, Kyungsan University
4Laboratory of Histology, College of Veterinary Medicine, Kyungpook National University |
| 백급수침이 HCl-aspirin으로 유발된 십이지장 궤양에 미치는 조직학적 및 면역조직화학적 연구 |
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구세광1, 이형식2, 김종대3, 이재현4 |
1(주)동화약품 중앙연구소 약리독성연구실
2경산대학교 자연과학부 생물학전공
3경산대학교 한의과대학
4경북대학교 수의과대학 조직학교실 |
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| Abstract |
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To identify, the effects of acua-acupuncture of Bletillae rhizoma extract on the duodenal ulcer induced by HCl-aspirin in rats, the changes of histological profiles, number of mucous-producing goblet cells (PAS-positive cells), and morphology and/or number of chole-cystokinin (CCK)-8 and serotonin-immunoreactive (IR) cells in the gastrointestinal tract were observed after acua-acupuncture of Bletillae rhizoma extract on Ganshu locus ($BL_{14}$). Samples were collected at 1, 3 and 5 days after treatment. Histologically, very severe injury, atrophy of villus, necrosis of epithelial cell and hemorrhage, to the duodenal mucosa including epithelium were observed in HCl-aspirin administrated control groups, and these injuries were increased with time intervals. But in acua-acupuncture groups and nontreated normal groups, no gross lesion of ulcer was demonstrated and histologically minor injury to the apex of villas epithelium was observed. Compared to the PAS-positive cells of the control groups, those of the acua-acupuncture groups were increased. Severe degranulation of CCK-8- and serotonin-IR cells were observed in the control groups but these phenomena seldom occur in the acua-acupuncture groups. Serotonin-IR cells were significantly decreased in control groups but increased in the acua-acupuncture groups compared to control groups. However, these degranulation of IR cells of the aqua-acupuncture groups were less severe than those of normal groups, and number of serotonin-IR cells were lower than those of normal groups but these phenomena were decreased with time intervals and in 5 days after treatment, they were observed like those of normal groups. These result indicated that acua-acupuncture of Bletillae rhizoma extract would be accelerated the healing of the duodenal ulcer but the functional mechanisms were unknown. |
| Key Words:
duodenal ulcer, histology, acua-acupuncture, Bletillae rhizoma extract, immunohistochemistry |
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