Intracellular Mg<sup>2+</sup> concentration dependent Mg<sup>2+</sup> release in the hearts

Baek, Sung-soo; Kim, Shang-jin; Kim, Jln-shang

Korean Journal of Veterinary Research 2000;40(2):291-299.

Intracellular Mg²⁺ concentration dependent Mg²⁺ release in the hearts

Sung-soo Baek, Shang-jin Kim, Jln-shang Kim

College of Veterinary Medicine, Chonbuk National University

심장에서 세포내 Mg²⁺ 농도 의존적 Mg²⁺ 유리

백성수, 김상진, 김진상

전북대학교 수의과대학

Abstract

Magnesium ($Mg^{2+}$) transport across the plasma membrane of cardiac myocytes appears to be under hormonal control. Repeated stimulations with adrenergic or histaminergic agonist produced a progressive decrease in $Mg^{2+}$ efflux from hearts. Thus we hypothesized that the $Mg^{2+}$ efflux may be resulted from a down-regulation of receptors or from a depletion of $Mg^{2+}$ from intracellular pool(s) in the hearts. In the present study, the regulation of $Mg^{2+}$ homeostasis by receptor stimulation was studied in perfused rat and guinea pig hearts. The successive short addition of norepinephrine (NE) to rat and guinea pig, and of histamine (HT) to perfused guinea pig hearts induced a progressive decrease in $Mg^{2+}$ efflux. These $Mg^{2+}$ effluxes were blocked by propranolol or ranitidine, respectively. These decrease in $Mg^{2+}$ efflux were inhibited by sodium cyanide (NaCN), which increases intracellular $Mg^{2+}$ ($[Mg^{2+}]_i$) levels. When NE (or HT) was added after HT (or NE), this efflux was also decreased in the guinea pig hearts. In the rat hearts and myocytes, HT did not stimulate $Mg^{2+}$ efflux. But NE produced a large $Mg^{2+}$ efflux after stimulation with HT. 8-(4-Chlorophenylthio)-adenosine cAMP (cAMP), like NE and HT, also induced a progressive decrease in $Mg^{2+}$ efflux in guinea pig hearts. This effect was inhibited by NaCN. These data provide evidence that the progressive decrease in receptor-stimulated $Mg^{2+}$ efflux is considered to be due to a decrease in $[Mg^{2+}]_i$ levels rather than receptor down-regulation.

Key Words: magnesium, histamine, intracellular $Mg^{2+}$, norepinephrine, heart