Korean Journal of Veterinary Research 2000;40(4):701-706.
In vitro selection of lactic acid bacteria for probiotic use in pigs
Jae-won Byun1, Gyung-tae Kim2, Hyoung-suk Bae2, Voung-jin Baek2, Wan-kyu Lee1
1College of Veterinary Medicine, Chungbuk National University
2Korea Yakult Ltd. R & D Center
양돈용 생균제 개발을 위한 유산균주 선발
변재원1, 김경태2, 배형석2, 백영진2, 이완규1
1충북대학교 수의과대학
2(주)한국야쿠르트 중앙연구소
This study was carried out to select the lactic acid bacteria(Lactobacillus, Streptococcus and Bifidobacterium) and yeast for probiotic use in pigs. Acid-tolerant 536 strains were isolated from the feces of 30 pigs. To select useful strains, the first screened strains were treated with strong acid solution(pH 2.5 to 3.0) for 3 hours and subsequentely treated with the anaerobic diluent solution containing 0.15% Oxgall for 3 hours. Among these strains, 151 strains showed strong tolerance to both acid and bile. Lactobacillus and Streptococcus tolerant to the acid and bile were treated with heat at $80^{circ}C$ for 15 min, and at $70^{circ}C$ for 5 min in Bifidobacterium and yeast. As a result of heat treatment, 38 strains were obtained as heat-tolerant strains. All of heat-tolerant strains were tested for antibiotic resistance against virginiamycin, sulfathiazole, aureomycin, neomycin, linsmycin, tiamulin and ASP250 which were used as feed additives for growth promotion in pigs. Finally, one strain each from Lactobacillus, Streptococcus, Bifidobacterium and yeast that showed resistance to acid, bile, heat and antibiotics was selected for probiotic use in pigs.
Key Words: probiotics, lactic acid bacteria, acid and bile tolerance, heat tolerance, antibiotic resistance

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