| Determination of new anti-HIV agents, the KR-V series, in rat plasma using microbore high-performance liquid chromatography |
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Young-mi Lee1, Myung-jin Park1, Jin-suk Kim2, Ho-chul Shin1 |
1Department of Pharmacokinetics & Toxicokinetics, Korea Research Institute of Chemical Technology
2College of Veterinary Medicine, Konkuk University, Korea |
| HPLC를 이용한 랫드혈장내 새로운 항HIV제 KR-V series의 분석법 |
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이영미1, 박명진1, 김진석2, 신호철1 |
1한국화학연구소 약물독성동태연구실
2건국대학교 수의과대학 |
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| Abstract |
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We have developed a rapid, simple and precise high-performance liquid chromatographic (HPLC) method using an UV detection system for the determination of new anti-HIV candidates, nineteen KR-V compounds, in rat plasma. We used a analytical columnn of $C_{18}$ ($5{mu}m$, $250{ imes}2.0mm$ I.D.) and a mobile phase of water and ACN mixture (40/60, v/v). Under these conditions, all the KR-V compounds were readily separated from plasma with retention times of 4-12 min. The limits of quantitation for the 19 KR-V compounds were 15-30 ng/ml. The recoveries from the plasma were higher than 85% (C.V.<10%) with exception of KR-V 2, 7 and 15. The compounds KR-V 2, 7 and 15, containing ester moieties, were found to be unstable in plasma. This result suggests that esters, like KR -V 2, 7 and 15, should be excluded from future structure design studies of anti-HIV KR-V agents. In conclusion, the current HPLC method is a valuable analytical tool for investigating the pharmacokinetics of the KR-V series in rats. |
| Key Words:
HIV, HPLC, KR-V series, rat plasma |
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