| Development of ELISA for brucella abortus RB51 I. Analysis on antigens of Brucella abortus RB51 by Westeren blot |
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Moon Her, Dong-hee Cho, Byeong-yeal Jung, Seong-kun Cho, Suk-chan Jung, Ok-kyung Kim |
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Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Services |
| 부루세라 RB51의 ELISA 진단법개발 I. Westeren blot에 의한 Brucella abortus RB51균의 항원 분석 |
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허문, 조동희, 정병열, 조성근, 정석찬, 김옥경 |
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국립수의과학검역원 세균과 |
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| Abstract |
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As compared with reaction of antibody for sonicated antigen of Brucella abortus strain RB51 and 1119-3 by Western blot analysis, Brucella field positive sera was detected strong reaction at 40~80 kDa LPS of strain 1119-3, but detected very weak reaction at strain RB51 partly. Otherwise, as we analyzed major immunogen of RB51 by antisera bled periodically during 6 months after RB51 vaccination. we detected strong immunological reaction at 17, 18 and 8 kDa antigen of RB51. Especially, reaction of 8 kDa antigen by Western blot coincided with reaction of dot-blot assay in RB51-antibody detection method. We also compared with reaction of field sera by STAT(standard tube agglutination test), dot-blot assay and Western blot (reaction of 8 kDa antigen of strain RB51). 16 sera of 4~5 months after RB51 vaccination are all negative by STAT, and 12 field brucellosis positive serum are all positive, and also 12 of 16 sera vaccinated RB51 are positive by dot-blot assay and reaction of 8kDa antigen by Western blot. but 1 of 15 Brucellosis negative sera reacted nonspecifically dot-blot assay. |
| Key Words:
Brucella abortus, RE51, 8 kDa antigen |
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