Korean Journal of Veterinary Research 2001;41(2):203-210.
The apoptotic fragment assay in rat peripheral lymphocytes and crypt cells with whole body irradiation with 60Co ?-rays and 50 MeV cyclotron fast neutrons
Tae-hwan Kim
Laboratory of Radiation Effect, Korea Cancer Center Hospital
코발트-60 감마선과 50 MeV 싸이크로트론 고속 중성자선에 전신조사된 랫드의 말초 임파구와 음와 세포의 아포토시스 유도를 이용한 생물학적 선량 측정 모델 개발 연구
김태환
원자력 병원, 방사선 영향 연구실
Abstract
Here, we compared the effectiveness of 50 MeV($p{ o}RBe^+$) cyclotron fast neutrons versus $^{60}Co$ ${gamma}$-rays by the apoptotic fragment frequency in both rat peripheral lymphocytes and crypt cells to check a radiobiological endpoint. The incidence of apoptotic cell death was increased in all irradiated groups, and radiation at all doses trigger rapid changes in both crypt cells and peripheral lymphocytes. These data suggest that apoptosis may play an important role in homeostasis of damaged radiosensitive target organ by removing damaged cells. The curve of dose-effect relationship for these data of apoptotic fragments frequencies was $y=0.3+(6.512{pm}0.279)D(r^2=0.975)$ after neutrons, while $y=0.3+(4.435{pm}0.473)D+(-1.300{pm}0.551)D^2(r^2=0.988)$ after ${gamma}$-rays. In addition, $y=3.5+(118.410{pm}10.325)D+(-33.548{pm}12.023)D^2(r^2=0.992)$ after ${gamma}$-rays in rat lymphocytes. A significant dose-response relationship was found between the frequency of apoptotic cell and dose. These data show a trend towards increase of the numbers of apoptotic cells with increasing dose. Dose-response curves for high and low linear energy transfer (LET) radiation modalities in these studies were different. The relative biological effectiveness (RBE) value for crypt cells was 1.919. In addition, there were significant peaks on apoptosis induction at 4 and 6h after irradiation, and the morphological findings of the irradiated groups were typical apoptotic fragments in crypt cells that were hardly observed in the control group. Thus, apoptosis induction in both crypt cells and peripheral lymphocytes could be a useful endpoint of rat model for studying screening test and microdosimetic indicator to evaluate the biological effects of radiation-induced cell damage.
Key Words: Intestinal crypt cell, peripheral lymphocytes, apoptotic fragment assay, low dose radiation, biodosimetric animal model
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