Occurrence of postweaning multisystemic wasting syndrome in pigs in Jeju

Kang, Jong-chul; Jeong, Kyong-ju; Ahn, Mee-jung; Lee, Du-sik; Kang, Wan-chul; Kim, Jin-hoe; Shin, Tae-kyun

Korean Journal of Veterinary Research 2001;41(3):367-371.

Jong-chul Kang¹, Kyong-ju Jeong¹, Mee-jung Ahn¹, Du-sik Lee¹, Wan-chul Kang², Jin-hoe Kim², Tae-kyun Shin¹

¹Department of Veterinary Medicine, Institute for Life Science, Brain Korea 21, Cheju National University
²Animal Health Department of Jeju Provincial Institute for Livestock Promotion

제주지역 양돈장 자돈에서 발생한 이유후 전신성 소모성 증후군의 증례

강종철¹, 정경주¹, 안미정¹, 이두식¹, 강완철², 김진회², 신태균¹

¹제주대학교 수의학과
²제주도 축산진흥원 가축위생시험과

Abstract

Multiplex PCR and immunohistochemistry were used to detect and differentiate between porcine circovirus (PCV) type-I and the PCV associated with postweaning multisystemic wasting syndrome (PMWS). Unique DNA product to PCV type-II was confirmed the some organs including lymph nodes, tonsil and spleen from eight pigs in Jeju by multiplex PCR. In this study, the samples were tested by a multiplex PCR assay to determine the type of PCV in each case; all cases were PCV type-II positive. PCV type-II was identified not only in typical PMWS cases, but also in field cases submitted with various clinical histories, some of which were not suggestive of PMWS. Immunohistochemically PCV type-II antigen was detected in macrophage-like cells in the tonsil, liver, lymph nodes and spleen, while some hepatocytes and renal tubular epithelial cells were also positive to the virus. This study suggested that PCV type-II is one of the causative agents of PMWS as well as the major type of PCV in the affected pigs in Jeju.

Key Words: porcine circovirus, polymerase chain reaction, immunohistochemistry