Korean Journal of Veterinary Research 2001;41(3):381-385.
Establishment of diagnositc method for bovine neosporosis by PCR using paraffin block
Jung-keun Lee1, Jae-Hoon Kim2, Jin-Hyun Kim1, Byung-chun Lee1, Woo-suk Hwang1, Hee-jeong Youn1, Hoo-woo Nam3, Young-wha Jean2, Dae-Yong Kim1
1College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
2National Veterinary Research Institute and Quarantine Service
3Department of Parasitology, College of Medicine, Catholic University
파라핀 블록 PCR을 이용한 소 네오스포라 감염증의 진단법 확립
이중근1, 김재훈2, 김진현1, 이병천1, 황우석1, 윤희정1, 남호우3, 진영화2, 김대용1
1서울대학교 수의과대학, 농생명공학부
2국립수의과학검역원
3카톨릭의과대학 기생충학교실
Abstract
Neospora caninum infections have been associated with neonatal paresis as well as abortion around the world. Bovine abortion induced by N caninum was first reported in 1997 in Korea. Diagnosis of N caninum infection is usually based on histopathology and immunohistochemical detection of organism. However, often the tissues having lesion suggestive of N caninum infection were negative on immunohistochemistry. Here, we describe establishment of PCR-based diagnostic strategy for N caninum infection using DNA extracted from paraffin blocks containing the lesion. PCR was able to amplify N caninum-specific bands from the paraffin blocks containing at least moderate degree of inflammation. Compared to paraffin-blocks, DNA extracted from fresh tissues were less sensitive than that of paraffin blocks. This PCR-based method can be practically applicable for rapid diagnosis of bovine N caninum infection with high specificity and sensitivity. Based on this method, 17% of bovine abortion surveyed during a designated period was associated with N caninum infection.
Key Words: bovine, Neospora caninum, PCR, diagnosis, abortion


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