| Establishment of diagnositc method for bovine neosporosis by PCR using paraffin block |
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Jung-keun Lee1, Jae-Hoon Kim2, Jin-Hyun Kim1, Byung-chun Lee1, Woo-suk Hwang1, Hee-jeong Youn1, Hoo-woo Nam3, Young-wha Jean2, Dae-Yong Kim1 |
1College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
2National Veterinary Research Institute and Quarantine Service
3Department of Parasitology, College of Medicine, Catholic University |
| 파라핀 블록 PCR을 이용한 소 네오스포라 감염증의 진단법 확립 |
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이중근1, 김재훈2, 김진현1, 이병천1, 황우석1, 윤희정1, 남호우3, 진영화2, 김대용1 |
1서울대학교 수의과대학, 농생명공학부
2국립수의과학검역원
3카톨릭의과대학 기생충학교실 |
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| Abstract |
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Neospora caninum infections have been associated with neonatal paresis as well as abortion around the world. Bovine abortion induced by N caninum was first reported in 1997 in Korea. Diagnosis of N caninum infection is usually based on histopathology and immunohistochemical detection of organism. However, often the tissues having lesion suggestive of N caninum infection were negative on immunohistochemistry. Here, we describe establishment of PCR-based diagnostic strategy for N caninum infection using DNA extracted from paraffin blocks containing the lesion. PCR was able to amplify N caninum-specific bands from the paraffin blocks containing at least moderate degree of inflammation. Compared to paraffin-blocks, DNA extracted from fresh tissues were less sensitive than that of paraffin blocks. This PCR-based method can be practically applicable for rapid diagnosis of bovine N caninum infection with high specificity and sensitivity. Based on this method, 17% of bovine abortion surveyed during a designated period was associated with N caninum infection. |
| Key Words:
bovine, Neospora caninum, PCR, diagnosis, abortion |
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