| Diagnosis of swine sarcoptic mange(Sarcoptes scabiei var. suis) by ELISA |
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Cha-Ho Jee, Sam-sun Lee, Lai-hun Chang |
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Lab of veterinary parasitology, College of Veterinary Medicine, Chungbuk National University |
| ELISA를 이용한 돼지 옴 (Sarcoptes scabiei var. suis) 감염증의 진단 |
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지차호, 이삼선, 장래훈 |
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충북대학교 수의과대학 수의기생충학교실 |
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| Abstract |
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The diagnosis of swine sarcoptic mange (Sarcoptes scabiei var. suis) was investigated by ELISA in order to replace current diagnostic methods such as skin scraping, scratching index, or lesion score of dermatitis. The current methods need many efforts and much times and cost much. They can not handle many samples simultaneously. Therefore, in this research we developed ELISA that can handle many samples at a time. The antigens of swine sarcoptic mite were isolated and examined by 12.5% SDS-PAGE and silver staining. The antigenicity of antigen was confirmed by Western blotting using the swine from the artificailly-infested swine with swine sarcoptic mite. The optical density (OD) values of the artificailly-infested positive sera and the naturally-infested positive sera of sows were measured and read in order to confirm the stability of antigens, the reproducibility and validity (sensitivity and specificity) of the manufactured ELISA of swine sarcoptic antigens. In above results, the developed ELISA would be possible to use the diagnostic tool of sarcoptic mange if OD values of piglets, fattening pigs and sows are interpreted reasonably and classified as mange-free and infested. |
| Key Words:
ELISA kit, swine sarcoptic mange (Sarcoptes scabiei var. suis), sensitivity and specificity of ELISA, mange-free pig farm |
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