Induction of micronuclei in human, rabbit and dog lymphocytes irradiated in vitro with gamma radiation |
Si-yun Ryu1, Bit-na Kang1, Ho-jun Kim1, Tae-hwan Kim2, Kyu-sik Jeong3, Se-ra Kim4, Hae-june Lee4, Sung-ho Kim4, Mi-Young An5 |
1College of Veterinary Medicine, Chungnam National University 2Korea Cancer Center Hospital 3College of Veterinary Medicine, Kyungpook National University 4College of Veterinary Medicine, Chonnam National University 5College of Veterinary Medicine, Texas A&M University |
사람, 토끼 및 개 유래 말초혈액 림프구의 미소핵을 이용한 방사선 피폭의 생물학적 선량측정 |
류시윤1, 강빛나1, 김호준1, 김태환2, 정규식3, 김세라4, 이해준4, 김성호4, 안미영5 |
1충남대학교 수의과대학 2한국원자력병원 3경북대학교 수의과대학 4전남대학교 수의과대학 5Texas A&M University 수의과대학 |
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Abstract |
The frequencies of gamma-ray-induced micronuclei (MN) in cytokinesis-blocked (CB) lymphocytes at several doses were measured in three donors of three species (human, rabbit, dog). Measurements performed after irradiation showed a dose-related increases in MN frequency in each of the donors studied. When analysed by linear-quadratic model the line of best fit was : human : $y=0.1184D+0.01867D^2+0.01$, rabbit : $y=0.0387D+0.00528D^2+0.01$ (y = number of MN/CB cells and D = irradiation dose in Gy). The relative sensitivity of rabbit lymphocytes compared with human lymphocytes was estimated by best fitting linear-quadratic model based on the radiation-induced MN data over the range from 0 Gy to 4 Gy. In the case of MN frequency with 0.2, the relative sensitivities of rabbit lymphocytes was 0.39. These data indicate that the induction of MN in rabbit CB cells following irradiation was much less sensitive to the MN induction effects of gamma-irradiation than those from human. The MN assay with dog lymphocytes was very difficult and time-consumed because the dog PHA-stimulated lymphocytes yielded cultures with very low level of CB cells formation in the condition of this experiment. Our in vitro radiobiological study confirmed that the cytogenetic response obtained in blood from rabbit can be utilized for application in environmental studies. |
Key Words:
gamma-radiation, micronuclei, human, rabbit, dog, lymphocyte |
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