| NPY Distribution in Diencephalon of Developing Chick |
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Mee-Young Lee1, Il-Kwon Park1, Kyoung-Youl Lee1, Hyo-Jung Kwon1, Chi-Won Song1, Mee-Sun Park1, Kang-Iee Lee2, Kwon-Soo Ha3, Won-Sik Kim4, Moo-Kang Kim1 |
1College of Veterinary Medicine, Chungnam National University
2College of Oriental Medicine, Taejon University
3Korea Basic Science Institute
4College of Medicine, Chungnam National University |
| 발생중인 병아리의 사이뇌에서 NPY 양성반응세포의 분포 |
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이미영1, 박일권1, 이경열1, 권효정1, 송치원1, 박미선1, 이강이2, 하권수3, 김원식4, 김무강1 |
1충남대학교 수의과대학
2대전대학교 한의과대학
3기초과학지원연구소
4충남대학교 의과대학 |
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| Abstract |
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This study deals with the distribution and morphological study of the neurons with the neuropeptide Y (NPY) in developing chick brain. The developing brains of Korean native chicks at embryonic days 8 (E8), E10, E12, E14, E16, E18, and E20 were used. The chicks were perfused with 4% paraformaldehyde through the left ventricle and aorta. The brains were removed and transferred into 30% sucrose, and then cut in a cryostat into $60{mu}m$ in thickness. The sections were immunostained with free-floating and avidin-biotin complex (ABC) methods. The numerous neurons with NPY were first observed in nucleus rotundus of diencephalon at E8. In particular, neurons in nucleus rotundus had the well-developed processes. At E12, the neurons with NPY were distributed widely in diencephalon; nucleus septalis lateralis, medialis, nucleus magnocelluaris preopticus dorsalis, medialis, ventralis, nucleus preopticus medialis, nucleus dorsolateralis anterior thalami pars magnocellularis, and nucleus paraventricularis magnocellularis (paraventricular nucleus) except nucleus rotundus. From E12 to E20, positive neurons were oval-shaped, changed gradually into the spherical- and multipolar-shaped. The shapes of processes were also omnidirectional and the number of those were less than in telencephalon. As the chicks developed, the morphology of neurons with NPY showed the tendency to increase in their sizes and numbers. |
| Key Words:
NPY, diencephalon, chick, development |
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