| Prevalence of paratuberculosis of dairy cattle in Kangwon area |
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Doo Kim1, Kwan-joon Jeon1, Jong-taek Kim1, Kwang-soon Shin2, Myung-kyun Shin3, Guk-hyun Chang3, Jeung-ki Kim3, Och-sung Kim3, Jae-young Jung3 |
1Department of Veterinary Medicine, Kangwon National University
2College of Veterinary Medicine, Chungnam National University
3Kangwon Veterinary Service Laboratory |
| 강원지역 젖소의 요네병 감염실태 |
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김두1, 전관준1, 김종택1, 신광순2, 신명균3, 장국현3, 김정기3, 김옥성3, 정재영3 |
1강원대학교 수의학과
2충남대학교 수의과대학
3강원도 가축위생시험소 |
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| Abstract |
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The purpose of this study was to conduct diagnosis of bovine paratuberculosis in Kangwon area. Blood samples were collected from 2,261 dairy cows of 162 herds, and the ELISA and immunoblotting using recombinant 34KDa protein of M. paratuberculosis were conducted. The feces collected from dairy cows were cultured on HEY medium with mycobactin-J and PCR was conducted with washing solution of medium 4 weeks after culture. The ELISA had sensitivity of 83.3% and specificity of 96.7%. And the immunoblotting had sensitivity of 83.3% and specificity of 100%. Of the 2,261 dairy cows, 371 cows(16.4%) were positive in ELISA and 75 cows(3.3%) were positive in immunoblotting. And of the 162 herds, 109 herds(67.3%) had an apparent paratuberculosis prevalence by ELISA and 40 herds(24.7%) by immunoblotting. The geographic distribution of herds with paratuberculosis was not uniform. Of the 241 feces samples including 110 feces from ELISA positive cow, 9 feces were positive in culture and PCR. PCR was able to detect the growth of M. paratuberculosis as early as 4 weeks of culture. |
| Key Words:
Bovine paratuberculosis, recombinant 34KDa protein, diagnosis, Kangwon area |
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