| Optimalization of ELISA using Recombinant p27 Protein of SIV for Detection of Anti-SIV |
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Eun-ok Kim, Eun Kim, Yoon-i Oh, Kwang-soon Shin, Hyun-soo Kim, Chul-joong Kim |
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College of Veterinary Medicine, Chungnam National University |
| SIV의 p27 재조합 단백질을 이용한 SIV 항체 검출을 위한 ELISA의 최적 조건 |
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김은옥, 김은, 오윤이, 신광순, 김현수, 김철중 |
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충남대학교 수의과대학 |
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| Abstract |
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The p27 coding region of the SIVmac239 isolate was amplified by PCR and cloned into an expression vector, pMAL-cri, which expressed high levels of the p27 protein from Escherichia coli. The purified p27 protein was used for detection of anti-SIV antibodies with the sera from 11 macaques and 21 marmosets by immunoblot assay of which one macaque was suspicious for the SIV infection. The optimum conditions of ELISA was studied by the check board system with the recombinant purified p27 protein. For the plate coating, 200ng/well of the purified p27 was satisfactory. The conjugate was diluted 1:1000. The sera from the 32 monkeys were negative for the anti-SIV by ELISA. |
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