Korean Journal of Veterinary Research 2002;42(2):153-162.
Effect of Alginic Acid on Experimentally Induced Arthropathy in Rabbit Model
Young-Hoon Bai1, Tae-Hun An1, Sung-Chul Lim1, Sok-Cheon Pak2, Jae-Chang Lee3, Nam-Hyun Kang4, Chun-Sik Bae5
1Chosun University Medical School
2Hamilton College of Oriental Medicine
3Environmental Department, Naju College
4K-1 Biotech
5College of Veterinary Medicine, Chonnam National University
토끼의 관절병증에 미치는 알긴산올리고당의 치료효과
배영훈1, 안태훈1, 임성철1, 박석천2, 이재창3, 강남현4, 배춘식5
1조선대학교 의과대학
2해밀튼 한의과대학
3나주대학 환경학과
4K-1 바이오텍
5전남대학교 수의과대학
For the induction of arthropathy, 5% hydrogen peroxide($H_2O_2$) was injected for 5 weeks into the intraarticular space of the New Zealand white rabbits to damage articular cartilage. Alginic acid of low molecular weight (2%) made from macromolecular alginate treated with enzyme was administered into articular space at the dose of 5 mg/kg twice a week for 3 and 6 weeks using 1 ml syringe and 26 G needle. Saline was injected for the control. Tissues surrounding the articulation were obtained for the measurements of superoxide dismutase(SOD) activity as a major antioxidant enzyme and malondialdehyde (MDA) as a lipid peroxidation level. Histopathologic examination on the surface of articular cartilage was carried out. Data showed that injection of hydrogen peroxide for 5 weeks had led to the induction of free radical damage and of articular cartilage change as confirmed by microscopic observation. The application of hydrogen peroxide caused a gradual increase in the SODs and MDA. These patterns were similar after 3 and 6 weeks of alginate treatment. Furthermore, microscopic examinations revealed that hydrogen peroxide caused flaking, fibrillation, fissuring, denudation, and hypocellularity in the articular surfaces. In conclusion, lipid peroxidation was demonstrated in the articular cartilage by the administration of hydrogen peroxide in the rabbit model. This lipid peroxidation could be caused by oxygen free radicals. The histologic and enzymatic correlations on lipid peroxidation in the articulation have provided a better understanding of arthropathy. It is possible to take advantage of these findings to evaluate effective alginate dosage more efficiently.
Key Words: Arthropathy, alginic acid, superoxide dismutase activity, lipid peroxidation

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