| Matrix solid phase dispersion isolation and high performance liquid chromatographic determination of five benzimidazole anthelmintics in bovine muscle, liver and omasum |
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Chung-Hui Kim1, Gon-Sup Kim1, Jung-Hee Park1, Dae-Sik Hah2, Jae-Doo Ryu3, Sung-Gi Son4, Jung-Ho Heo5, Myung-Ho Jung5, Jong-Shu Kim1 |
1College of Veterinary Medicine, Gyeongsang National University(Institute of Animal Medicine)
2Gyeongnam Provincial Government Institute of Health and Environment
3National Veterinary Research and Quarantine Service Pusan Regional Office
4Gyeongnam Livestock Promotion Institute
5Gyeongnam Livestock Promotion Institute South-branch |
| 시료고체상분산처리와 액체크로마토그라피를 이용한 소의 근육, 간 및 천엽에서의 벤지미다졸계 구충제 잔류분석 |
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김충희1, 김곤섭1, 박정희1, 하대식2, 류재두3, 손성기4, 허정호5, 정명호5, 김종수1 |
1경상대학교 수의과대학(동물의학연구소)
2경상남도 보건 환경 연구원
3국립수의과학 검역원 부산지원
4경상남도축산진흥연구소
5경상남도 축산진흥연구소 남부지소 |
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| Abstract |
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Simultaneous multiresidue analysis using liquid chromatography determination for five benzimidazole anthelmintics(thiabendazole, oxibendazole, albendazole, mebendazole and fenbendazole) in bovine muscle, liver and omasum has been described. Blank or benzimidazole-fortified samples(0.5g) were blended with bulk $C_{18}$($40{mu}m$, 18% load, endcapped, 2g). A column made from the resultant $C_{18}$/animal tissue matrix was first washed with hexane($8m{ell}$), following which the benzimidazoles were eluted with acetonitrile($8m{ell}$). Analytes of extracted sample were determined by liquid chromatography with UV detector at 290nm. Correlation coefficients of standard curves for individual benzimidazole isolated from fortified samples, using internal standardization, were linear($0.991{pm}0.007$ to $0.996{pm}0.005$) with average relative percentage recoveries from $62.1{pm}3.8(%)$ to $92.3{pm}7.5(%)$ for the concentration range($0.2{sim}6.4{mu}g/g$), respectively. Recoveries rates of TBZ, MBZ in liver, OBZ, MBZ in muscle and TBZ, MBZ in omasium from fortified benzimidazole were 92.%, 87.3%, 74.5%, 82.7%, 75.2% and 83.5% at condition II, respectively. Condition II showed higher recoveries rates than condition I. These results indicated that the matrix solid phase dispersion(MSPD) methodology is acceptable for the determination of 5 benzimidazole anthelmintics and may also suitable for other matrixes of food animal origin. |
| Key Words:
benzimidazoles, MSPD, liquid chromatography, bovine muscle, liver, omasum |
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