Antiparasitic Effect of Diminazene Aceturate on Theileria sergenti Types in Calves

Baek, Byeong-Kirl; Lee, Young-Jun; Kim, Byeong-Soo

Korean Journal of Veterinary Research 2002;42(2):261-268.

Byeong-Kirl Baek¹, Young-Jun Lee¹, Byeong-Soo Kim²

¹Biosafety Research Institute, Chonbuk National University
²Department of Clinical Pathology, Sohae College

Diminazene aceturate의 Theileria sergenti types에 대한 구충 효과

백병걸¹, 이영준¹, 김병수²

¹전북대학교 생체안전성연구소
²서해대학 임상병리과

Abstract

Bovine piroplasmosis caused by Theileria sergenti results in economic loss in the dairy industry. The majority of calves infected with T. sergenti in Korea harbor mixed populations with Buffeli, Chitose and Ikeda types. The T. sergenti types of the infected calves were examined to evaluate the effects of diminazene aceturate on their infection. To confirm the type of the T. sergenti infection, the allele-specific PCR was performed with the erythrocyte specimen from the 5 naturally infected calves. The dfferent allele-specific genes encoding the p32, p33 and 34, the immunodominant piroplasmin surface proteins, were amplified using the 3 sets of the oligonucleotide primers by PCR. The calves were treated with diminazene aceturate at the dose of 2mg/kg once intravenously and monitored for 12 months at one month intervals by the allele-specific PCR. Diminazene aceturate did not effect the Ikeda type infection. But diminazene aceturate effected the Chitose and Buffeli type infection reducing T. sergenti parasitemia. It is postulated that diminazene aceturate may effect the infection of the Chitose and Buffeli types, but not that of Ikeda type.

Key Words: Theileria sergenti, Allele-specific PCR, diminazene aceturate, Cattle