Korean Journal of Veterinary Research 2002;42(3):335-349.
Study on Immunological Response on Salmonella gallinarum in Immunosuppressed Chickens
Gye-Yeup Kim1, Jae-Hyang Lim2, Hong-Bum Koh2, Kyoung-Oh Cho2, Tae-Youl Kim1, Myoung-Hwa Oh1
1College of Oriental Medicine, Dongshin University
2Research Institute of Veterinary Medicine, Chonnam National University
면역억압계군에서 Salmonella gallinarum 감염에 대한 면역반응
김계엽1, 임재향2, 고홍범2, 조경오2, 김태열1, 오명화1
1동신대학교 한의과대학
2전남대학교 동물의학연구소
The immune response against Salmonella gallinarum infection was investigated in immunosuppresed chickens. Newly hatched chickens were treated with cyclophosphamide at the first and second day after birth and were challenged intraperitoneally with S gallinarum ($1{ imes}10^7CFU/m{ell}$) on day 6. Group 1, Immunosuppresed and Challenged group, was treated with cyclophaiphamide and challenged with S gallinarum; group 2, Immunosuppressed group, was only treated with cyclophsphamide; group 3, Challenged group, was only challenged with S gallinarum; group 4, Control group. In each group, the localization of lymphocytes of the lymphoid organs and intestine was immunohistochemically compared using a variety of monoclonal antiboies ($CD4^+$, $CD8^+$, and B lymphocyte). Also, S gallinarum were assessed by Maccallum-Goodpasture stain and immunohistochemical analysis in the paraffin-embedded intestinal tissues. In S gallinarum challenged chickens, $CD4^+$ and $CD8^+$ lymphocytes of the intestinal organs such as duodenum, jejunum, ileum and colon were increased. However, in cyclophophamide treated chickens, $CD4^+$ and $CD8^+$ lymphocytes and especially B lymphocytes of the lymphoid organs such as thymus, spleen, and bursa of Fabricius were dramatically decreased. These results suggest that cyclophsophamide is an immunosuppressive agent that especially causes depletion of B lymphocytes, suppress humoral immunity and eventually suppresses avian immune responses. Its protection against S gallinarum infection is mainly dependent on both cell-mediated mechanism and the humoral immune response.
Key Words: Salmonella gallinarum, cyclophosphamide, $CD4^+$, $CD8^+$, B cell

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