A case of parentage testing in the Thoroughbred horse by microsatellite DNA typing

Cho, Gil-Jae; Yang, Young-Jin; Kim, Bong-Hwan

Korean Journal of Veterinary Research 2003;43(1):25-29.

Gil-Jae Cho¹, Young-Jin Yang¹, Bong-Hwan Kim²

¹Equine Blood Typing Laboratory, Korea Racing Association
²College of Veterinary Medicine, Kyungpook National University

Microsatellite DNA형에 의한 더러브렛 말의 친자감정예

조길재¹, 양영진¹, 김봉환²

¹한국마사회 혈액형검사실
²경북대학교 수의과대학

Abstract

This study was carried out to investigate a usefulness of the microsatellite DNA markers for parentage verification of Thoroughbred (TB) horses. 9 TB horses samples were genotyped for nine international minimum standard markers (AHT4, 5, ASB2, HMS3, 6, 7, HTG4, 10, and VHL20), and the additional panel of four markers, ASB17, CA425, LEX33, and TKY321. This methods consisted of multiplexing PCR procedures, and it showed reasonable amplification of all PCR products. Genotyping was performed with an ABI 310 genetic analyzer. Foal I was excluded according to principles of Mendelian genetics in AHT4 (H/K), ASB2 (Q/Q), HMS3 (I/P), HTG4 (M/O), HTG1O (K/R), VHL20 (M/P), ASB17 (F/N), LEX33 (M/O), and TKY321 (G/I) markets. Foal II was excluded with markers AHT5 (K/M), ASB2 (M/N), HMS7 (N/N), HTG1O (K/K), VHL20 (I/I), ASB17 (F/F) and TKY321 (G/I). Foal III was excluded with markers AHT4 (O/O), AHT5 (K/K), ASB2 (M/R), HMS6 (M/P), HMS7 (O/O), HTG10 (R/S), VHL20 (L/M), and ASB17 (N/O). These results suggest that the present DNA typing is so useful for parentage verification of TB horses.

Key Words: microsatellite, parentage verification, thoroughbred horse