| Epidemiological Investigation of Staphylococcus aureus Isolated from Bovine Mastitis Based on the Polymorphism of Coagulase Gene |
|
Jin-San Moon1, Ae-Ri Lee2, Suk-Kyung Lym1, Yi-Seok Joo1, Hyun-Mi Kang1, Jong-Man Kim1, Mal-Nam Kim2 |
1National Veterinary Research and Quarantine Service
2Department of Biology, Sangmyung University |
| 젖소 유방염 유래 Staphylococcus aureus의 Coagulase Gene 유전형 분석에 의한 감염경로 규명 |
|
문진산1, 이애리2, 임숙경1, 주이석1, 강현미1, 김종만1, 김말남2 |
1국립수의과학검역원
2상명대학교 |
|
|
| Abstract |
|
Because Staphylococcus aureus (S. aureus) has variable number of short sequence repeat region in coagulase gene, it has been used to investigate the relatedness of S. aureus isolates. In this study, we isolated S. aureus strains from 20 dairy farms with bovine mastitis from September 2000 to August 2001. PCR-RFLP analysis of coagulase gene revealed 10 different patterns. Most of the S. aureus isolates showed only one coagulase gene RFLP pattern per farm. However, there were several S. aureus clones spreading between dairy farms. All the farms showed poor management conditions of milking machine and milker, indicating that managements for mastitis control program include use of proper milking matching, premilking sanitation, and segregation in the S. aureus infection herd. Our data suggest that PCR-RFLP analysis of coagulase gene might be applicable for the epidemiological investigations of S. aureus isolated from bovine mastitis cows. |
| Key Words:
cow, mastitis, Staphylococcus aureus, coagulase gene |
|
PDF Links
Full text via DOI
Download Citation
