Effect of high concentrations of carboxymethyl chitosan on preventing formation of peritoneal adhesion in rats

Kwon, Young-Sam; Jang, Hwan-Soo; Jeong, Jae-Hoon; Bae, Jae-Sung; Rhee, Jeong-Woo; Kang, Han-Saem; Yang, Jeong-Hoon; Li, Wen-Xue; Jang, Kwang-Ho

Korean Journal of Veterinary Research 2003;43(1):151-156.

Young-Sam Kwon, Hwan-Soo Jang, Jae-Hoon Jeong, Jae-Sung Bae, Jeong-Woo Rhee, Han-Saem Kang, Jeong-Hoon Yang, Wen-Xue Li, Kwang-Ho Jang

College of Veterinary Medicine, Kyungpook National University

랫드에서 고농도의 Carboxymethyl Chitosan이 복벽 유착 예방에 미치는 영향

권영삼, 장환수, 정재훈, 배재성, 이정우, 강한샘, 양정훈, 이문학, 장광호

경북대학교 수의과대학

Abstract

The objective of this study was to determine the effective concentration of carboxymethyl chitoaan(CMC) solution on preventing intraperitoneal adhesions. In this study, 60 rats were divided into four groups : an untreated control group and three experimental groups that were treated with 3 ml of 3, 5, and 7% CMC solution. Adhesions were induced by suturing both the ileal serosa and peritoneum scraped until petechial bleeding occurred. CMC solutions were instilled intraperitoneally immediately before closure of the peritoneomuscular layer. The tensile strength of formed adhesions was measured by using a computerized tensiometer. Histopathological changes of adhesions were evaluated by hematoxylin-eosin for light microscopic examination and Massons's trichrom staining for collagen fibers. All of CMC solutions reduced significantly the tensile strength of the adhesions. CMC inhibited the increase of inflammatory cell infiltration and damage of adhesion spot, and accelerated the collagen synthesis and neovascuiarization. No significant differences were observed among all of CMC concentration at inhibiting adhesion formation. Therefore, we could conclude that 3% CMC, the lowest concentration adopted in this study, was appropriate to prevent the formation of peritoneal adhesion.

Key Words: carboxymethyl chitosan, peritoneal adhesion, rat, tensile strength