Korean Journal of Veterinary Research 2003;43(2):219-230.
Genetic Characteristics of Porcine Epidemic Diarrhea Virus Isolated in Korea
Yong-zhe Chi1, Hyuk-moo Kwon1, Hyun-kyu Jeong2, Jeong-hee Han1
1Department of Veterinary Medicine, Kangwon National University
2Dodram Pig Farmers' Co-operative
돼지 유행성 설사 바이러스 국내분리주의 유전학적 특성 규명
지영철1, 권혁무1, 정현규2, 한정희1
1강원대학교 수의학과
2도드람 양돈조합
Abstract
Porcine epidemic diarrhea virus(PED), a member of Coronaviridea, is the etiological agent of enteropathogenic diarrhea in swine. The purpose of this study was to investigate genetic characteristic of PEDV isolated in Korea. Nucleocapsid(N) gene and membrane (M) gene of recent Korean PEDV strains isolated in 2001 were amplified, cloned, sequenced and analyzed. N gene of seven Korean PEDV field isolates bad 94.5% to 99.4% nucleotide and 92.4% to 99.4% amino acid sequence homology each other. Nucleotide and amino acid sequences of Korean field PEDVs were different from published foreign PEDVs, showing 95.1% to 98.0% nucleotide and 93.5% to 97.6% amino acid sequence homology. By phylogenetic tree analysis on based nucleotide sequences, PEDVs were clustered into four groups. By phylogenetic tree analysis based on amino acid sequences. PEDVs were clustered into five groups. M gene of our Korean PEDV field isolates had 99.6% to 100% nucleotide and 98.7% to 100% amino acid sequence homology each other. Nuclotide and amino acid sequences of Korean field PEDVs were different from published foreign PEDVs, showing 98.5% to 98.8% nucleotide and 97.3% to 97.8% amino acid sequence homology. By phylogenetic tree analysis based on nucleotide and amino acid sequences, PEDVs were clustered into two groups which were Korean PEDV isolate group and foreign PEDV isolate group.
Key Words: PEDV, N gene, M gene, genetic characteristics, Korea


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