Detection of Antibodies to Classical Swine Fever Virus gp55 in Muscle Fluid

Jung, Jae-yun; Jung, Byeong-yeal; Kim, Bong-hwan

Korean Journal of Veterinary Research 2003;43(2):263-270.

Jae-yun Jung¹, Byeong-yeal Jung², Bong-hwan Kim¹

¹College of Veterinary Medicine, Kyungpook National University
²National Veterinary Research & Quarantine Service

Classical Swine Fever Virus gp55 항원에 대한 Muscle Fluid 항체 측정

정재윤¹, 정병렬², 김봉환¹

¹경북대학교 수의과대학
²국립수의과학검역원

Abstract

The objective of the present study was to investigate the use of fluid released from muscle samples as an alternative to serum for ELISA to detect classical swine fever(CSF) virus antibodies in slaughter pigs. The optimal correspondence between serum 1:20 OD values and muscle fluid OD values was achieved at a muscle fluid dilution of 1:2. Significant correlation was found between serum and neck muscle ELISA ($r_s=0.880$, p<0.0001, ${kappa}=0.82$; specificity of 97.0% and sensitivity 90.6%). The semimembranous muscle showed similar correlation in CSF ELISA($r_s=0.877$, p<0.0001, ${kappa}=0.75$; specificity of 94.1% and sensitivity 89.1%). High correlation was obtained between serum and mesenteric lymph node in the CSF ELISA ($r_s=0.937$, p<0.0001, ${kappa}=0.87$; specificity of 97.1% and sensitivity 93.0%). Measmement agreement between serum ELISA and muscle fluid ELISA was calculated and expressed as limits of agreement. The correspondence of ELISA of serum and muscle fluid indicated limits of agreement. Above 95% of all muscle fluid values were distributed within this limits of agreement. Among the samples used for ELISA for detecting CSFV antibodies, mesenteric lymph node had the most correlation and agreement with serum ELISA. F-test for comparison of variances showed no significant difference between the serum and muscle fluid. In conclusion, muscle fluid is a useful postmortem alternative to serum to detect CSFV antibodies.

Key Words: classical swine fever(CSF), hog cholera(HC), muscle fluid, ELISA