| Expression of VP2 of Aquatic Birnavirus GC-1 Isolated from Rockfish (Sebastes schlegeli), Rearing in Seawater in Korea |
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Seong-joon Joh1, Haan-woo Sung1, Yun-jeong Lee1, Jae-hong Kim1, Shien-young Kang2 |
1Avian Disease Division, National Veterinary Research & Quarantine Service
2College of Veterinary Medicine, Chungbuk National University |
| 국내 해산양식어 조피볼락에서 분리된 수생버나바이러스 GC-1의 VP2 발현 |
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조성준1, 성환우1, 이윤정1, 김재홍1, 강신영2 |
1국립수의과학검역원
2충북대학교 수의과대학 |
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| Abstract |
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The VP2 gene of aquatic birnavirus, Korean isolate (GC-1) was cloned and expressed using the baculovirus expression system. The VP2 gene and VP2 partial gene, which contained a neutralizing epitope, were constructed for recombinant transfer vectors, for baculovirus expression. The expressed recombinant proteins were confirmed by indirect immuno fluorescence antibody (IFA), SDS-PAGE and Western blot. The level of expression was checked at regular time using IFA and Western blot. To measure the neutralizing activity of recombinant proteins against GC-1 strain, the antisera against recombinant proteins were produced by using guinea pigs. The result showed that the antisera neutralized the GC-1 strain. However, the neutralizing titer was higher in antisera against the VP2 gene expressed recombinant protein than that of VP2 partial gene recombinant protein. |
| Key Words:
aquatic birnavirus, GC-1, VP2, expression |
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