| Apoptosis of Germ Cells after Vasectomy in Rats |
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Jong-yun Choi1, Sung-whan Cho1, Si-yoon Ryu1, Young-heun Jee2, Geun-jwa Lee3, Hwa-young Son1 |
1College of Veterinary Medicine, Chungnam National University
2Department of Veterinary Medicine, Cheju National University
3Chungnam Livestock & Veterinary Service Institute |
| 수컷 랫드에서 졍관절제술에 의한 생식세포의 Apoptosis |
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최종윤1, 조성환1, 류시윤1, 지영흔2, 이근좌3, 손화영1 |
1충남대학교 수의과대학
2제주대학교 수의학과
3충남축산위생연구소 |
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| Abstract |
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The pathological mechanism of impaired spermatogenesis after vasectomy has not been completely investigated. In this study, we examined pathological changes of the testis and the Fas-Fas ligand (FasL) mediated signaling pathway in apoptotic germ cell death after vasectomy in rats. Ten-weeks old Sprague-Dawley rats were underwent bilateral vasectomy and sacrificed after 1 day, 2 days, 3 days, 5 days, 1 week, 2 weeks, and 4 weeks of surgery and the testes were removed. Histopathological evaluation of spermatogenesis was performed by hematoxylin-eosin and periodic acid-Schiff-hematoxylin staining. To elucidate the pathophysiology of seminiferous tubule damage, terminal dUTP nick end labeling staining, electrophoresis assay of DNA fragmentation, and Western blotting analysis for Fas-FasL were performed. Relative weights of testes were decreased from 5 days after vasectomy. Germ cell degeneration were first found in the spermatogonia and spermatocytes at stages I-VI, and XII-XIV seminiferous tubules. Mean incidence of apoptotic germ cells after vasectomy progressively increased to peak in 5 days, and then gradually decreased to the control levels in 2 weeks after vasectomy. The expression of Fas-FasL reached maximum level at 5 days after vasectomy and then declined. In conclusion, impaired spermatogenesis after vasectomy associated with an increase in germ cell apoptasis, which is partly mediated by the activation of Fas-FasL. |
| Key Words:
vasectomy, spermatogenesis, apoptasis, Fas-Fas ligand |
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